Microporation was performed 2, 5, and 10 times on each sample. ; Bos, G.W. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. Proc. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. Curvale, R.; Masuelli, M.; Perez Padilla, A. Monolayers and Thin Films of Dextran Hydrophobically Modified. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. ; Grishchenko, A.E. Accessibility These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. Clipboard, Search History, and several other advanced features are temporarily unavailable. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. Gillespie, T.; Hulme, M. A. The genus Weissella: taxonomy, ecology and biotechnological potential. Ahn, G.Y., Eo, HS., Kim, D. et al. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Carbohydrate Polymers 72, 356-360. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Biomaterials. ; Prasad, D.H.L. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. (1969). In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. 4K, b M.W. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. American Journal of Food Science and Technology 2013, 1(3), 60-66. Granath, K. A. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Google Scholar. The human skins were from the back or thigh of a 24-year-old woman. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. eCollection 2015. Analytical Chemistry. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. Comparison of single-point and dilution procedures. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Chem. (2001). The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth The measurements of dextrans molecular weight were determined by size exclusion HPLC (Gilson, France) with Refraction Index detector using a Polysep-GFC-P5000 column (7.8030 mm). Webof overall macromolecular geometry and hydrodynamic draining. Intrinsic viscosity-molecular weight relationship for chitosan. II. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Sachdeva V, Banga AK. TEWL measurements were taken 30min after microporation of the human and pig skin. The .gov means its official. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. Colloid and Polymer Science, 286, 1223-1231. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). Dextran Fractions. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. WebDextran 40 Pharmaceutical Quality. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. 2019;9(3-S):9726. (1987). Search All authors read and approved the final manuscript. government site. Biomacromolecules 8, 2464-2475. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC They concluded that a power law behavior seems to be approached only in the small molecular weight region. European Polymer Journal, 36, 2143-2146. The reason for a nonlinear Mark-Houwink relationship is confirmed by the intrinsic viscosity which in contrast with scaling prediction shows no power law behavior. Please enable it to take advantage of the complete set of features! and transmitted securely. (2013). Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Theory, Estimation, Experiment, and Data. Before WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Molecular weight (Mv) and hydrodynamic properties can be calculated from there. On the molecular weight distribution of dextran T-500. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). Viswanath, D.S. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th National Library of Medicine The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. ; Bataille, B. 4K and M.W. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Would you like email updates of new search results? Synthesis and characterization of blue dextrans. dextran hydrodynamic radius. J Cosmet Laser Ther. Formation of dextran hydrogels by crystallization. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). European. The basic setup of a DLS instrument is shown in Figure 1. ; Rjumtsev, E.I. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. Carbohydrate Polymers, 36, 319-326. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Microporation applications for enhancing drug delivery. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. An official website of the United States government. Biomaterials, 22, 1891-1898. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. (2007). J Pharm Sci. (2006). Carbohydrate Polymers, 38, 267-271. Part of The Viscosity of Dilute Solutions of Long-Chain Molecules. 2004;25(9):155967. Pure Appl. (2003). what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. The micropore formed at the SC through to the epidermis, enabling drug delivery. Pharm Res. Progr. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Masuelli, M. A. Eremeeva, T. E.; Bykova, T. O. Keywords: FITC dextran; intestinal permeability; transit time. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.
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